A tissue separating device (100) is provided. The tissue separating device (100) includes a canister device (20) including a canister body (21) defining a volume. A tissue retrieval port (36) can be arranged on the canister device (20) and is capable of being arranged in fluid communication with a harvesting device (300) for directing a fatty liposuction aspirate into the volume of the canister device (20). An adjustable height filtration mesh assembly (50) can be arranged within the canister body (21) and can include a filtering mesh (62). A tissue harvesting port (24) can be arranged in the sidewall of the canister body (21) and can be capable of being arranged in communication with a collection device (400) to allow the tissue harvesting port (24) to atraumatically receive a filtered pure fat collected on the filtering mesh (62). The filtration mesh assembly (50) can be movably arranged within the canister body (21) such that the filtering mesh (62) is adjustable with respect to the tissue harvesting port (24).
