The application provides Methods of characterization of Metabolic Profiles, Profiles and Profiles of phenotypic traits in plants or groups of plants. In addition, the application also provides methods to establish an objective Model between a phenotypic profile and Metabolic Profile, profile or between a trait and Metabolic Profile.In addition, also provided are methods of using such models aim to predict accurately the Development a phenotype of interest or a feature of interest in a separate Floor, immature.In fulfilment of the request, a Target Model is established using the phenotypic Profiles and Metabolic Profiles of at least two groups of plants, where groups of Plants Exhibit different phenotypes or grow under different environmental conditions.Alternatively, a Target Model can be established using the profiles of features and Metabolic Profiles of at least two groups of plants, where groups of Plants Exhibit different features or grow under different environmental conditions.In such embodiments, the target models of the application can be determined using various combinations of partial least-squares analysis, Partial least-squares Discriminant analysis, Principal Components analysis, cross validation, variable importance for Ch. Projection calculations, Support Vector Machines and Neural Networks.The Metabolic Profiles of plants or groups of Plants covered by the application can be characterized, for example, using Chromatography and Mass spectroscopy.In a specific implementation, the load Mass Fragments detected by Mass Spectrometry, including the Metabolic Profiles of the application are not identified, characterized, or otherwise reported prior to Statistical Analysis. Thus, the Metabolic Profiles of the application include the complete set of Metabolites detected.Also provide methods to predict the development of a phenotype or trait of interest in a Plant not used for establishing the objective model of the application, i.e., on a separate floor.In On
