Donald E. INGBER,Michael SUPER,Jeffrey Charles WAY,Mark J. CARTWRIGHT,Julia B. BERTHET,Dinah R. SUPER,Martin ROTTMAN,Alexander L. WATTERS
申请号：
US16683630
公开号：
US20200239552A1
申请日:
2019.11.14
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.
