Wolf B. Frommer,Sakiko Okumoto,Loren Looger,Marcus Fehr
申请号：
US11665339
公开号：
US08530633B2
申请日:
2005.10.14
申请国别(地区):
US
年份:
2013
代理人:
摘要：
Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.
