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РАСТВОР ДЛЯ ФИКСАЦИИ БИОЛОГИЧЕСКИХ КЛЕТОК
专利权人:
НОВАСИ (FR)
发明人:
ПЕЛЬТЬЕ Эрик (FR)
申请号:
RU2012136482/13
公开号:
RU2012136482A
申请日:
2011.01.20
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. The fixing solution for storing in vitro cytological sample containing nucleated cells and erythrocytes comprising an alcohol to fix cells with saline solution to prevent osmotic shock to the cell wall level, formalin and polyethylene glycol to maintain the size and integrity of the nucleated cells and erythrocytes, said fixed solution, the fixing solution is characterized in that it contains no acetone or class of compounds ketones, or acetic acid to maintain the integrity of the erythrocytes ov.2. The fixing solution according to claim 1, characterized in that the alcohol is ethanol or izopropanolom.3. The fixing solution according to claim 1, characterized in that the alcohol is a mixture of ethanol and izopropanola.4. The fixing solution according to claim 1, characterized in that the quantity of alcohol is significantly lower than 45% by volume of the fixing rastvora.5. The fixing solution according to claim 1, characterized in that the amount of formalin is between substantially 0.2 to about 1% by volume of the fixing rastvora.6. The fixing solution according to claim 1, characterized in that it comprises a buffer providing a pH of the fixing solution is substantially 6.4 to 7,4.7. The fixing solution according to claim 1, characterized in that it contains from 80% to 95% by volume: 590 ml saline, 10 ml of polyethylene glycol (Carbowax®), 203 ml of isopropyl alcohol, 193 ml of pure ethanol, 0.01% by volume sodium azide, and 20% to 5% by volume buffered 4% formalin.1. Фиксирующий раствор, предназначенный для сохранения in vitro цитологического образца, содержащего ядерные клетки и эритроциты, содержащий спирт для фиксации клеток, физраствор для предотвращения осмотического шока на уровне клеточной стенки, формалин и полиэтиленгликоль для сохранения размера и целостности ядерных клеток и эритроцитов, фиксированных упомянутым раствором, причем фиксирующий раствор характеризуется тем, что он не содержит ни ацетона или соединений класса кетонов, ни уксусной кисл
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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