PURPOSE: A manufacturing method of a natural preservative and sterilizer is provided to manufacture a non-smelling and non-toxic natural preservative by using an aspergillus mite removing medicinal herb and plant.CONSTITUTION: A manufacturing method of a natural preservative and sterilizer comprises a step of putting a preservative using a natural medicinal herb and a aspergillus mite removing medicinal herb into a caldron and boiling the caldron for long time and manufacturing 200g of a concentrate solution a step of manufacturing 200g of a concentrate solution by mixing 1000g of a cut and dried sweet flag root with 1000g of a rhododendron leaf, and putting the same with 10 times water into the caldron a step of manufacturing a shiso leaf oil by extracting oil from a shiso leaf and manufacturing the oil to a preservative a step of mixing 150kg of a food raw material, 20g of the shiso fruit oil and using the shiso fruit seed oil as a preservative when manufacturing a food from the shiso fruit liquid a step of using an agricultural chemical to remove aspergillus and mite by mixing 1000 ml of water, the rhododendron leaf solution in the second solution.COPYRIGHT KIPO 2013본 발명은 천연 약초인 차조기 및 그조성물을 이용한 누룩곰팡이 진드기제거제 제조방법에 관한 것으로 차조기 잎과 줄기를 물과 일정한 비율로 혼합하여 오래 끓여서 차조기 농축액을 제조 하고 또한 차조기 씨앗으로 기름을 짜서 상기 차조기 농축액과 기름을 식품원료에 일정한 비율로 혼합하여 천연방부제로 사용하는 방법과 석창포와 만병초잎을 물에 끓여서 농축한 용액을 누룩곰팡이 와 진드기를 제거 할 수 있는 천연 농약제조방법에 관한 기술을 제공한 것이 특징이다.