Linear genetic construct and methods for generating a transgenic or progeny plant resistant to ssdna virus and a genetically modified plant or part thereof
FARMACULE BIOINDUSTRIES PTY LTD.;QUEENSLAND UNIVERSITY OF TECHNOLOGY;QUTBLUEBOX PTY LTD.
发明人:
BENJAMIM DUGDALE,DOUGLAS KENNETH BECKER,GREG JOHN FAFNER,JAMES LANGHAM DALE,ROBERT MAXWELL HARDING,SCOTT RICHARD HERMANN,SRIMEK CHOWPONGPANG
申请号:
BR0109522
公开号:
BRPI0109522B1
申请日:
2001.03.28
申请国别(地区):
BR
年份:
2015
代理人:
摘要:
"construct, method of generation of a transgenic or progeny plant thereof, resistant to ssdna virus, linear genetic element and avian species and genetically modified plant or part thereof". The present invention relates generally to constructs and, in particular, to genetic constructs comprising polynucleotide sequences capable of releasing, in a circular, covalently closed form, from a larger nucleotide sequence such as, but not limited to. limitation, a genome of a eukaryotic cell. preferably, once released, a polynucleotide sequence is reconstituted into a form that allows expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence for all or part of a foreign nucleotide such as, but not limited to, an intronic sequence or other junction signal inserted therein. expression and, in particular, coding sequence transcription involves joining the strange sequence. release and circularization is generally in response to a stimulus such as a protein mediated stimulus. more particularly, the protein is a prokaryotic or eukaryotic cell or virus derived protein or developmentally and / or tissue specific regulated protein. The construct of the present invention is particularly useful for conferring resistance against pathogens or apoptosis induction or other mechanisms of cell death useful, for example, in treating cancer or inducing male or female sterility in plants. The constructs therefore allow site specific expression and / or developmentally regulated expression of selected genetic sequences."construto, método de geração de planta transgênica ou progenia da mesma resistente a vírus ssdna, elemento genético linear e espécie aviária e planta geneticamente modificadas ou parte da mesma". a presente invenção relaciona-se, em geral, a construtos e, em particular, a construtos genéticos que compreendem seqüências de polinucleotídeos capazes de liberar, em forma circular, covalentemente fechada, a pa