PROBLEM TO BE SOLVED: To irradiate a local area of a living body such as a prescribed region of the hand with light and to excellently acquire an living body transparent image of the local area of the living body.SOLUTION: The intravital observation device includes: a surface light emitting part 2 which has a light emitting surface 201 facing a living body placement area X and irradiates the living body in the living body placement area X with light an imaging part 3 for receiving light transmitting the living body in the living body placement area X and outputting image data and a light shield member 8 which is provided between the living body placement area X and the light emitting surface 201 of the surface light emitting part 2 and shields a part of the light emitted from the light emitting surface 201.COPYRIGHT: (C)2012,JPO&INPIT【課題】手の所定部位等の生体の局所領域に対して光を照射できるようにするとともに、当該生体の局所領域の生体透視画像を好適に取得できるようにする。【解決手段】生体配置領域Xを向く発光面201を有し、生体配置領域Xにある生体に対して光を照射する面発光2部と、生体配置領域Xにある生体を透過した光を受光して画像データを出力する撮像部3と、生体配置領域Xと面発光部2の発光面201との間に設けられ、当該発光面201から出る光の一部を遮る遮光部材8とを具備する。【選択図】図7