Grains Research and Development Corporation;Commonwealth Scientific and Industrial Research Organisation
发明人:
Morell, Matthew Kennedy,Ral, Jean-Philippe Francois Michel,Li, Zhongyi
申请号：
AU2008329557
公开号：
AU2008329557B2
申请日:
2008.11.27
申请国别(地区):
AU
年份:
2014
代理人:
摘要：
The specification provides methods of obtaining a genetically modified plantwhich has increased production potential compared to a control plant, the methodcomprising the steps of i) obtaining a plurality of plants at least one of whichcomprises in its genome a heterologous polynucleotide, ii) identifying fromthe plurality of plants a plant which has increased production potential relativeto the control plant and comprises the heterologous polynucleotide, and iii)selecting the genetically modified plant, wherein the polynucleotide comprisesa transcriptional control sequence operably linked to a nucleic acid sequencewhich encodes an agent that modifies endogenous starch phosphorylation and/orstarch degradation in the plant. In some embodiments, the plant has increasedendogenous glycosylase or increased digestibility compared to a control plant.In some specific embodiments, the endogenous starch phosphorylation and/orstarch degradation is modified by modifying expression or activity of one ormore enzymes selected from the group consisting of α-amylase (EC 3.2.1.1),β-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase(EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase(EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, waterdikinase (GWD, EC 2.7.9.4).
