A culture equipment with constant temperature and which prevents the occurrence of vapour on the wall is disclosed. The culture equipment includes at least two transparent structures to form a wall and a hermetic space is formed between the two transparent structures. A low thermal conductivity substance is filled up the hermetic space. Besides, the culture equipment includes a temperature controller.一種恆溫及防結露之生物飼養裝置,具有至少兩層透明結構之一壁體,於該至少兩層透明結構之間形成一密封之空間,並填充一低熱傳導介質至該密封之空間中;該恆溫及防結露之生物飼養裝置更包括一溫度控制單元。1‧‧‧缸體11、12‧‧‧透明結構13‧‧‧空間14‧‧‧生物生長空間15‧‧‧底座16‧‧‧上框17‧‧‧上蓋171‧‧‧孔洞18‧‧‧燈源