The present invention relates to the pemetrexed preparation comprising nonaqueous solvents, when which keeps stabilization at least about 48 small when storing at 2 DEG C to 8 DEG C after dilution. The invention further relates to the pemetrexed preparation comprising nonaqueous solvents, which keeps stablizing at least about 24 months when storing at 2 DEG C to 8 DEG C.