The present disclosure relates to a method for producing a therapeutic composition containing mammalian cells, which can be used for producing the method, which comprises dispersing the mammalian cells with about 3% to about 10% glucose aqueous solution or Ringer's acetate solution. Provided are a cell preparation, a treatment method using the therapeutic composition, and the like.本開示は、哺乳動物細胞を含む治療用組成物の製造方法であって、哺乳動物細胞を約3%~約10%のブドウ糖水溶液または酢酸リンゲル液で分散させることを含む方法、その製造に用いうる細胞製剤、前記治療用組成物を用いた治療方法等を提供する。