FRAUNHOFER-GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
发明人:
申请号：
ARP120101980
公开号：
AR086674A1
申请日:
2012.06.05
申请国别(地区):
AR
年份:
2014
代理人:
摘要：
This provides a method for the generation of a Plant Cell Line from a heterologous monoclonal population of plant cells, which comprises the following steps: (a) the provision of a population of cells of heterologous plants； (b) the preparation of protoplast S of this population of cells of heterologous plants； (c) the separation of single protoplasts.The preparation of protoplasts subjected to the selection of Flow Cytometry； (d) the regeneration of protoplast transformed until the formation of a separate individual microcolonia through co Culture in the presence of cellular material of food； (e) the removal of MICR Ocolonia Cell material of foodAnd the cultivation of microcolonia until the formation of a monoclonal Cell Line Plant.Claim 1: a method for the generation of a Plant Cell Line from a heterologous monoclonal population of plant cells, which comprises the following steps: (a) the provision of a population of cells of heterologous plants； (b) the preparation of protoplast S of this population of cells of heterologous plants； (c) the separation of single protoplasts.The preparation of protoplasts subjected to the selection of Flow Cytometry； (d) the regeneration of protoplast transformed until the formation of a separate individual microcolonia through co Culture in the presence of cellular material of food； (e) the removal of MICR Ocolonia Cell material of foodAnd the cultivation of microcolonia until the formation of a monoclonal Cell Line Plant.La presente provee un método para la generación de una línea celular de planta monoclonal a partir de una población heteróloga de células de plantas, que comprende las siguientes etapas: (a) la provisión de una población heteróloga de células de plantas； (b) la preparación de protoplastos a partir de dicha población heteróloga de células de plantas； (c) la separación de protoplastos individuales, sometiendo la preparación de protoplastos a la selección de citometría de flujo； (d) la regeneración de un protoplasto
