Embodiments of the invention provides a method for detection of at least one analyte derived from a sample, comprising the steps of: a) depositing the sample on a surface of a solid support b) transferring at least a portion of the solid support to a receptacle suitable for performing a specific binding assay for one or more analytes of interest c) optionally washing the portion d) adding a single specific binding partner for each analyte of interest to the receptacle, the binding partner being labelled with an oligonucleotide sequence e) mixing the portion with nucleic acid amplification reagents f) amplifying the oligonucleotide sequence and g) detecting amplified nucleic acid. The invention also provides a kit for use with the method for detection of at least one analyte derived from a sample.