In some embodiments, images of a cornea may be obtained, and the images of the cornea may be segmented to detect a tear film layer of the cornea and an epithelium layer of the cornea. Thickness of the tear film layer and thickness of the epithelium layer may be determined based on the segmentation of the images of the cornea. A thickness map may be generated based on the thickness of the tear film layer and the thickness of the epithelium layer. As an example, the thickness map may comprise visual differences in thickness across the tear film layer and the epithelium layer. In some embodiments, the foregoing may be performed with respect to one or more other microlayers of the cornea in addition to or alternatively to the tear film layer or the epithelium layer.