[Object] An object of the present invention is to provide an automatic blood-sampling tube preparation system which can reduce the reading mistake of the information of the RFID tags. [Means of Realizing the Object] An automatic blood-sampling tube preparation system according to the present invention being constituted so as to selectively take out a blood-sampling tube required to an examination of a patient according to a doctor's instruction information from the blood-sampling tube containing section, write the patient examination information on a RFID tag of the taken out tube on the basis of the instruction information, read simultaneously all patient examination information from the all RFID tags contained in the prepared container for every patient, compare the information read from the RFID tags with the instruction information. The automatic blood-sampling tube preparation system takes the prepared tray move so as to change the state of the RFID tags contained in the container and performs the reading process and comparing process.