The present invention provides a method for calibrating a working plane of a medical detection apparatus, which is used for calibrating the working plane of the medical detection apparatus to be parallel with a first reference plane, wherein the working plane has a first point to be calibrated and a second point to be calibrated thereon, the first point to be calibrated is located on the first reference plane, and the first point to be calibrated and the second point to be calibrated are respectively supporting points of a first foot and a second foot for supporting the working plane on the working plane, the above method comprising: receiving at least one first inclination angle value from an angle measuring tool, wherein each first inclination angle value is an angle between a line connecting the first point to be calibrated and the second point to be calibrated and the first reference plane; computing a vertical distance between the second point to be calibrated and the first reference plane as a first magnitude of adjustment according to a pre-stored distance between the first point to be calibrated and the second point to be calibrated and the first inclination angle value; and adjusting a height of the second foot according to the first magnitude of adjustment to allow the second point to be calibrated to be located on the first reference plane.