An isolated monoclonal antibody or antigen-binding fragment that specifically binds to stage-specific embryonic antigen 4. The monoclonal antibody or antigen-binding fragment includes a heavy-chain CDR1 having the sequence of SEQ ID NO: 33 or SEQ ID NO: 40, a heavy-chain CDR2 having the sequence of SEQ ID NO: 34 or SEQ ID NO: 39, a heavy-chain CDR3 having the sequence of SEQ ID NO: 35 or SEQ ID NO: 41, a light-chain CDR1 having the sequence of SEQ ID NO: 36 or SEQ ID NO: 42, a light-chain CDR2 having the sequence of SEQ ID NO: 37 or SEQ ID NO: 43, and a light-chain CDR3 having the sequence of SEQ ID NO: 38 or SEQ ID NO: 44. Also disclosed is an anti-tumor method carried out by administering the above monoclonal antibody or antigen-binding fragment to a subject having a tumor that expresses stage-specific embryonic antigen 4. Further provided are nucleic acids encoding the above sequences and recombinant cells containing the nucleic acids.一種經分離的單株抗體或抗原-結合片段，其專一性結合至階段特異性胚胎抗原4。該單株抗體或抗原-結合片段包含一具有序列辨識編號：33或序列辨識編號：40的序列之重鏈CDR1、一具有序列辨識編號：34或序列辨識編號：39的序列之重鏈CDR2、一具有序列辨識編號：35或序列辨識編號：41的序列之重鏈CDR3、一具有序列辨識編號：36或序列辨識編號：42的序列之輕鏈CDR1、一具有序列辨識編號：37或序列辨識編號：43的序列之輕鏈CDR2，以及一具有序列辨識編號：38或序列辨識編號：44的序列之輕鏈CDR3。亦揭示的是一種抗-腫瘤的方法，其是藉由對一具有表現階段特異性胚胎抗原4的腫瘤之個體投予上面的單株抗體或抗原-結合片段來施行。進一步提供的是編碼上面序列的核酸以及含有該等核酸的重組型細胞。
