FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
发明人:
Thomas Rademacher
申请号:
US14370109
公开号:
US10638679B2
申请日:
2013.01.31
申请国别(地区):
US
年份:
2020
代理人:
摘要:
The present invention relates to the generation and cultivation of plant cell material in the form of a non-tissue multilayer cell pack and its use for the accumulation or harvesting of a desired product. In particular, the invention provides a method for the generation of plant cell material in the form of a medium-deprived, porous structured and non-tissue multilayer cell pack and for the subsequent maintenance of said cell pack, comprising the steps of (i) providing a cell pack by separating cells from a plant cell suspension culture, wherein the content of the liquid comprised by the cell pack is reduced and adjusted to correspond to a cell pack density between 0.1 and 0.9 g wet cell weight per cm3, thereby establishing the medium-deprived and porous structured nature of said cell pack, and (ii) incubating said medium-deprived and porous structured cell pack in a non-liquid environment under a relative humidity of 50 to 100%.