PROBLEM TO BE SOLVED: To provide a cancer cell separation apparatus capable of selectively eliminating a cancer cell, and to provide a method for producing the same.SOLUTION: A cancer cell separation apparatus includes: a first flow path； a second flow path located below the first flow path and extending in the direction parallel to a longitudinal direction of the first flow path； and a through-hole connecting the first flow path and the second flow path, and having a diameter less than a width of the first flow path and a width of the second flow path in a cross section perpendicular to the longitudinal direction of the first flow path and the second flow path.【課題】がん細胞を選択的に除去することができるがん細胞分離装置およびその製造方法を提供する。【解決手段】がん細胞分離装置は、第１流路と、第１流路の下方に位置し、第１流路の長手方向と平行な方向に延びる第２流路と、第１流路と第２流路とを接続し、かつ、第１流路および第２流路の長手方向と垂直な断面において、第１流路の幅および第２流路の幅以下の径を有する貫通孔と、を含む。【選択図】図１
