Kurkin Vladimir Aleksandrovich,Morozova Tatyana Vladimirovna
申请号：
RU20170146138
公开号：
RU2669162(C1)
申请日:
2017.12.26
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: chemistry.SUBSTANCE: invention relates to chemical and pharmaceutical industry and relates to a method for quantifying the amount of flavonoids in Siberian hawthorn leaves. Method comprises preparing water-alcohol extract from hawthorn leaves by extracting 1 g of crushed leaves with ethyl alcohol and determining the amount of flavonoids by differential spectrophotometry. Raw material is extracted once, and ethyl alcohol in a concentration of 70 % is used as an extractant. Amount of flavonoids is quantified at a wavelength of 392 nm in terms of 2ʺ-O-rhamnoside vitexin. Content of the amount of flavonoids in terms of 2ʺ-O-rhamnozide vitexin and absolutely dry raw materials are calculated by formula,where D is an optical density of the test solution; Dis an optical density of the solution of standard sample of 2ʺ-O-rhamnoside vitexin; m is a raw material mass, g; mis a mass of standard sample of 2ʺ-O-rhamnoside vitexin, g; W is mass loss when drying in percent.EFFECT: technical result is the increase
