GILLIGAN THOMAS BOYD,SHARPE JOHNATHAN CHARLES,VISHWANATH RAMAKRISHNAN,EVANS KENNETH MICHAEL,MORENO JUAN
申请号：
NZ63038813
公开号：
NZ630388A
申请日:
2013.03.04
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
A method of efficiently sorting a sperm sample in a particle sorting instrument comprises a) establishing a sheath fluid stream in the particle sorting instrument； b) flowing a sperm sample into the sheath fluid stream； c) identifying viable X-chromosome bearing sperm and/or viable Y-chromosome bearing sperm in the sperm sample； d) separating the viable X-chromosome bearing sperm and/or the viable Y-chromosome bearing sperm from the remainder of the sperm sample； e) collecting the viable X-chromosome bearing sperm and/or the viable Y-chromosome bearing sperm； f) determining one or more measured sorting parameters in the particle sorting instrument； g) establishing a minimum productivity threshold； h) establishing a minimum purity threshold； i) determining a sorting efficiency based on the measured sorting parameters determined while sorting； and j ) adjusting one or more instrument parameters to increase the sorting efficiency while maintaining the productivity above the minimum productivity threshold and purity above the minimum purity threshold.
