Provided is a method for inducing CD4 - CD8 + T cells having an antigen specific cytotoxic activity from pluripotent stem cells, comprising the steps of: (1) differentiating pluripotent stem cells to give a cell culture comprising CD4 - CD8 - T cells and CD4 + CD8 + T cells, (2) removing CD4 - CD8 - cells from the cell culture obtained in step (1), and (3) differentiating the CD4 + CD8 + cells in the cell culture into CD4 - CD8 + T cells.