David E. Anderson,Jasminka Bozic,Barthelemy Ontsouka
申请号：
US15991044
公开号：
US20180274044A1
申请日:
2018.05.29
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The present disclosure provides methods useful for determining levels of HCMV infection in host cells and, by extension, determining levels of neutralizing antibodies present in a sample. The present disclosure encompasses the recognition that HCMV viruses that have a fluorescent moiety permit detection of viral infection (e.g., by assessing fluorescence in cells after contacting the host cell with the virus). In some embodiments, levels of HCMV infection are determined by fluorescence detection where the virus has been preincubated with a test sample (e.g., a serum sample) from a subject. In some embodiments, the subject has been administered a candidate HCMV vaccine.
