The present invention provides a method of treating subjects with non-ischemic dilated cardiomyopathy by administering a therapeutically effective amount of an isolated population of allogeneic human mesenchymal stem cells. The present invention also provides a method of treating subjects with symptoms of aging frailty by administering a therapeutically effective amount of an isolated population of allogeneic human mesenchymal stem cells.本發明提供藉由投藥治療有效量之分離的同種異體人類間葉幹細胞群治療具有非缺血性擴張型心肌病之受試者的方法。本發明亦提供藉由投藥治療有效量之分離的同種異體人類間葉幹細胞群治療具有老化衰弱症狀之受試者的方法。