Method of preparation a collagen sponge involves the following steps: prepare a collagen gel, into the collagen gel is stiring air to obtain a collagen foam which is dried to yield a dry block of collagen sponge with three-dimensional structure. From the block of collagen sponges are separated parts of sponge with cavity a diameter greater than 0.75 mm and less than 4 mm.Method of preparation a collagen sponge involves the following steps: prepare a collagen gel, into the collagen gel is stiring air to obtain a collagen foam which is dried to yield a dry block of collagen sponge with three-dimensional structure. From the block of collagen sponges are separated parts of sponge with cavity a diameter greater than 0.75 mm and less than 4 mm.