A semen collection device includes a core member, a mounting unit, and a position sensing module. The core member defines an insertion space having an inlet at an end. The mounting unit includes a main body having a main portion and a receiving portion extending from an end of the main portion, a cover detachably installed on the receiving portion. The cover and the receiving portion cooperatively define a cylindrical chamber for fixedly receiving the core member, and further define an opening communicating with the chamber for exposing the end of core member having the inlet. The position sensing module is located in the main portion substantially close to the opening and configured to detect a variation of amplitude and frequency of a position of the core member.