It is a kind of can anti-secondary pollution cell culture detection case,It includes there is a culture detection case ontology,The culture detection case ontology is equipped with a culture dish and passes in and out gate,A culture dish stand is equipped in the culture detection case ontology,An I/O device is further provided on the culture detection case ontology; One detection device,The detection device is arranged in the what culture detection case ontology; One arm mechanism,The arm mechanism is arranged in the what culture detection case ontology,To be moved between the culture dish stand and detection device and pick and place culture dish; By being equipped with detection device and arm mechanism in the culture detection case ontology,So that culture dish is being put into the culture detection case ontology Hou to before completing culture,In the process all without further taking out,So as to effectively avoid pollution and the problem of secondary pollution,Can pass through the arm mechanism automatically completes all detection operations simultaneously,And then this creation is made to can reach the effect of production reliability is substantially improved and saves human cost.一種可防二次污染的細胞培養檢測箱,其包含有一培養檢測箱本體,該培養檢測箱本體上設有一培養皿進出閘門,該培養檢測箱本體內設有一培養皿置放架,該培養檢測箱本體上進一步設有一輸出入裝置;一檢測設備,該檢測設備設置於該培養檢測箱本體內;一手臂機構,該手臂機構設置於該培養檢測箱本體內,用以在該培養皿置放架與檢測設備之間移動並取放培養皿;藉由該培養檢測箱本體內設有檢測設備與手臂機構,俾使培養皿在放入該培養檢測箱本體後到完成培養前,過程中皆無需再取出,從而可有效避免污染與二次污染的問題,同時可透過該手臂機構自動化完成所有檢測作業,進而使本創作可達到大幅提升產品可靠度與節省人力成本之功效。