Tissue splitting biopsy devices and methods use a needle portion and a sheath portion that are configured such as to allow simultaneous sectioning of a tissue to produce two tissue samples with corresponding surfaces using motion of the needle portion relative to the sheath portion. Most preferably, the needle portion has a chisel blade tip and at least two cavities opposite to each other and at the same distance from the tip, while the sheath portion has a sharpened front edge.
