A floor assembly for livestock handling equipment features a pair frame members lying in a longitudinal direction and spaced apart from one another in a lateral direction transverse to said longitudinal direction, and at least one support tray connected to the frame members and spanning therebetween in the lateral direction. At least one mat is supported atop the support tray(s). The mat is made of rubber or other resilient material for improved footing and comfort of the livestock. The topside of each mat is flush with a topside of the frame members to provide an even floor surface across the full area floor area. The mat(s) are removable for cleaning, and open gaps around the support tray(s) are provided for drainage. Non-welded attachment enables convenient removal and replacement of the support trays in the event of rust or other damage.