PROBLEM TO BE SOLVED: To enable a fluorescence image corresponding to a fluorescent material being used to be formed in a more suitable mode, even in a situation where a plurality of types of fluorescent materials are selectively used.SOLUTION: Provided is an imaging system that comprises: a light source device that applies light containing wavelength band components of at least some excitation wavelengths onto each of the plurality of types of fluorescent materials including a first fluorescent material that emits fluorescence belonging to a near infrared wavelength band and a second fluorescent material that emits fluorescence belonging to a visible wavelength band; an optical system unit; and an imaging device. The imaging device comprises: a branching optical system that includes a dichroic film; a first imaging element that images light belonging to the near infrared wavelength band that has been separated by the dichroic film; and a second imaging element that images at least a portion of light belonging to the visible wavelength band that has been separated by the dichroic film. A fluorescence image of fluorescence emitted by the first fluorescent material is formed by the first imaging element, and a fluorescence image of fluorescence emitted by the second fluorescent material is formed by the second imaging element.SELECTED DRAWING: Figure 1【課題】複数種類の蛍光物質が選択的に使用されるような状況下においても、使用される蛍光物質に応じた蛍光像をより好適な態様で撮像可能とする。【解決手段】近赤外波長帯域に属する蛍光を発する第1の蛍光物質と、可視光波長帯域に属する蛍光を発する第2の蛍光物質と、を含む複数種類の蛍光物質それぞれについて、励起波長の少なくとも一部の波長帯域の成分を含む光を照射する光源装置と、光学系ユニットと、撮像装置と、を備え、撮像装置は、ダイクロイック膜を有する分岐光学系と、ダイクロイック膜により分離された近赤外波長帯域に属する光が結像する第1の撮像素子と、ダイクロイック膜により分離された可視光波長帯域に属する光のうち少なくとも一部が結像する第2の撮像素子と、を有し、第1の蛍光物質が発する蛍光の蛍光像は、第1の撮像素子により撮像され、第2の蛍光物質が発する蛍光の蛍光像は、第2の撮像素子により撮像される、撮像システム。【選択図】図1