Provided are an injection preparation which includes: an aqueous composition containing pemetrexed or a salt thereof, at least one antioxidant agent A which is selected from the group consisting of cysteine and a salt thereof, and of which the content is 0.001 mass% to 0.1 mass% with respect to the total mass of the aqueous composition, thioglycerol of which the content is 0.001 mass% to 0.1 mass% with respect to the total mass of the aqueous composition, and an aqueous solvent of which the content is greater than or equal to 50 mass% with respect to the total mass of the aqueous composition, and a container which seals the aqueous composition, in which the concentration of oxygen in gas within the container which seals the aqueous composition is less than or equal to 2.0 volume%, and a method for producing the injection preparation.
