A method for transferring a quantity of analytes, such as micro organisms antibodies/antigens, anti microbially acting substances, nucleotides, antibiotics, hormones DNA sequences, enzymes, organic material biological material or material of biological origin, terrain enriching supplements or selective supplements of cultivating land comprising at least stages of: predisposing a substantially homogeneous mixture of a predetermined initial quantity of at least an analyte and a liquid, obtaining a concentration value or known amount of the analyte in the mixture introducing into the mixture at least a collecting portion (3) of a sampling device (1) having a support body (2), the collecting portion (3) comprising a first portion (2a) of the support body (2) and a plurality of fibres (6) attached to and arranged on the first portion (2a) of the support body (2) by means of flocking, in order to define a flocked collecting portion (3) or a flocked tampon such as to collect a part of the mixture on the collecting portion (3) and extracting the collecting portion (3) of the sampling device (1) of the mixture retaining on the sampling portion (3) a predetermined known quantity of the mixture to be transferred.