PURPOSE: A separating apparatus for platelet rich plasma is provided to remarkably shorten the solidification rate of PRP and plasma and enhance fibrin extraction productivity by performing second centrifuge in an inserting state of the coagulation catalyst of glass material within the upper chamber of the PRP separating device.CONSTITUTION: A separating apparatus (20) for platelet rich plasma comprises a main body (21) which has inner space and divided the inner space into upper liquid chamber (22) and lower liquid chamber (23) an upper cover (25) which covers the upper liquid chamber a lower cover (26) that rises according to the inner periphery of the lower liquid chamber which outer periphery is adhered a liquid collecting unit (24) that is formed between the upper liquid chamber and the lower liquid chamber and has a fluid path (24a) which connects the upper liquid chamber and the lower liquid chamber in the center unit in a upper protruded structure and coagulation catalyst body (27) which is used as catalyst to rapidly coagulate the PRP and the plasma with a centrifuge by being inserted inside the upper liquid chamber.COPYRIGHT KIPO 2013본 발명은 자가 혈소판 농축물질(Platelet Rich Plasma) 분리장치, 이를 이용한 자가 혈소판 농축물질 분리 및 응고방법에 관한 것으로서, 상세하게는, 원심분리를 통해 혈액으로부터 분리된 자가 혈소판 농축물질(PRP)과 혈장을 빠르게 고형화시켜 피브린(fibrin)을 추출할 수 있는 응고 촉매체를 구비한 자가 혈소판 농축물질 분리장치, 이를 이용한 자가 혈소판 농축물질 분리 및 응고방법에 관한 것이다.