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HAPLOID EMBRYOGENESIS
专利权人:
STICHTING DIENST LANDBOUWKUNDING ONDERZOEK
发明人:
Kimberly Boutilier,Gerrit Cornelis Angenent,Mercedes Soriano Castan,Li Hui
申请号:
US15024296
公开号:
US20160212956A1
申请日:
2014.09.24
申请国别(地区):
US
年份:
2016
代理人:
摘要:
A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, B. oleracea, Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.
来源网站:
中国工程科技知识中心
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