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GENE ENCODING APPARATUS FOR SYNTHESIZING CELLULOSE
专利权人:
MIZUNO KOICHI;OJI PAPER CO LTD
发明人:
MIZUNO KOICHI,KATO TOMOHIKO,SATO SHIGERU,SHIBATA DAISUKE
申请号:
JP19980239998
公开号:
JP2000060568(A)
申请日:
1998.08.26
申请国别(地区):
日本
年份:
2000
代理人:
摘要:
PROBLEM TO BE SOLVED: To obtain the new subject gene comprising a DNA containing a specific amino acid sequence and encoding a cellulose-synthetic enzyme complex, and usable for production of the enzyme for synthesizing a cellulose useful as a raw material for a paper pulp, a raw material for a fiber, a roughage, a raw material for fuel or the like. SOLUTION: This gene is the new one encoding a protein substantially comprising an amino acid sequence of formula I or II, and composing a cellulose synthetic enzyme complex. The gene is useful for production of the enzyme for synthesizing a cellulose important for a raw material for a paper pulp, a raw material for a fiber, a roughage, a raw material for fuel or the like. The DNA is obtained by preparing a mRNA from an epicotyl of Phaseolus angularis, synthesizing a cDNA by using the obtained mRNA as a template, and making a cDNA library by a conventional method, infecting Escherichia coli with the obtained cDNA library, growing the infected Escherichia coli on an agar medium reacting the grown product with a primary antibody against the cellulose synthetic enzyme complex, and the secondary antibody labeled with an alkali phosphotase, and screening the labeled product by detecting the labeled product.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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