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ACTIVATED LEUKOCYTE COMPOSITION
专利权人:
МАКРОКЬЮЭ; ЛТД. (IL)
发明人:
ШИРВАН Митчел (IL),ШИНАР Эйлат (IL),ФРЕНКЕЛЬ Орит (IL),ЗУЛОФФ-ШАНИ Ади (IL),БУБИС Марина (IL),БЭЙН Эйлат (IL),ГИНИС Ирэн (IL)
申请号:
RU2011139016/15
公开号:
RU2011139016A
申请日:
2010.03.05
申请国别(地区):
RU
年份:
2013
代理人:
摘要:
1. A method of manufacturing an activated leukocyte composition, comprising: a. incubating a person’s white blood cells for a period of time so that the white blood cells transition from a resting state to a functionally active state b. leukocyte exposure to hypoosmotic shock c. adding to the leukocyte stage b, a physiologically acceptable saline solution in an amount sufficient to restore isotonicity; d. it is possible to contact a separate sample of human plasma with a coagulant to obtain serum, where white blood cells and plasma can be obtained from the same donor or from different donors; as well. possibly mixing stage white blood cells with serum of stage d.2. The method according to claim 1, characterized in that the incubation occurs at a temperature of from about 12 ° C to about 28 ° C for from about 90 minutes to about 2, 3, 4, 5, 6, 7, 8, or 12-20 part 3. The method according to claim 1, characterized in that the incubation occurs at a temperature of up to about 37 ° C for about 5-24 hours. The method according to claim 1, characterized in that the leukocytes are obtained from a donor having a blood group of 0, Rhesus negative, and plasma is obtained from a donor having a blood group of AB, Rhesus positive. A method according to any one of claims 1 to 4, characterized in that the hypoosmotic shock comprises contacting white blood cells with water, where the physiologically acceptable saline solution is a sodium chloride solution. The method according to claims 1 to 4, characterized in that it further includes the incubation of leukocytes in serum at a temperature of approximately 37 ° C. An activated leukocyte composition comprising: a) from about 40% to about 90% of granulocytes; b)1. Способ изготовления активированной лейкоцитарной композиции, включающий:a. инкубацию лейкоцитов человека в течение некоторого периода времени, чтобы лейкоциты перешли от состояния покоя к функционально активному состоянию;b. подвергание лейкоцитов гипоосмотическому шоку;c. д
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