ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ УЧРЕЖДЕНИЕ "НАУЧНО-ИССЛЕДОВАТЕЛЬСКИЙ ИНСТИТУТ ВАКЦИН И СЫВОРОТОК ИМ. И.И.МЕЧНИКОВА" РОССИЙСКОЙ АКАДЕМИИ МЕДИЦИНСКИХ НАУК (ФГБУ "НИИВС ИМ. И.И.МЕЧНИКОВА" РАМН)
发明人:
Мелик-Нубаров, Николай Сергеевич,Черникова, Елена Вячеславовна,Файзулоев, Евгений Бахтиерович,Никонова, Александра Александровна,Вишневецкий, Дмитрий Викторович,Максимова, Екатерина Дмитриевна,Марова,
申请号：
EA201501099
公开号：
EA201501099A3
申请日:
2015.10.08
申请国别(地区):
EA
年份:
2016
代理人:
摘要：
The invention relates to the field of chemistry of high-molecular compounds, molecular biology and medicine and can be used for gene therapy of hereditary, oncological and infectious diseases. A method for the synthesis of cationic amphiphilic block copolymers for transfection of eukaryotic cells, characterized by the use of pseudo-living radical polymerization methods and the fact that the structure of all synthesized block copolymers contains a cationic block of a weak polyamine - poly (N, N-dimethylaminoethyl methacrylate) and a hydrophobic block representing the polymer or oligomer of a branched or brush structure - poly (oligopropylene glycol acrylate), including at the first stage the preparation of a reversible transfer polymer agent chains based on N, N-dimethylaminoethyl methacrylate by polymerization of N, N-dimethylaminoethyl methacrylate in the presence of 0.01-0.001 mol / l of dinitrile of azoisobutyric acid and 0.1-0.01 mol / l of a solution of dithiobenzoate (cyto-isopentanoic acid-methylacetate or tritium solution of dithiobenzoate (cyto-isopentanoic acid)； (S, S'-bis- (methyl-2-isobutyrate) tritiocarbonate) agent reversible chain transfer, resulting in a polymer of N, N-dimethylaminoethyl methacrylate containing a tritiocarbonate or dithiobenzoate group, in the second stage, the polymer (0.1-0 , 01 mol / l) and dinitrile azoisomasily acid (0.01-0.001 mol / l) was dissolved in a mixture oligopropilenglikolakrilat / benzene (3: 7) and the polymerization is carried out at 80 ° C for 24-48 hours, the resulting block copolymer out of solution, followed by freeze-drying in vacuo. The resulting preparations of amphiphilic cationic copolymers showed high transfection activity as during transfectionИзобретение относится к области химии высокомолекулярных соединений, молекулярной биологии и медицины и может быть использовано для генной терапии наследственных, онкологических и инфекционных заболеваний. Предложен способ синтеза катионных амфифильных блок-сополи
