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PROCÉDÉ D'ÉVALUATION DE LA QUALITÉ D'UNE RÉTINE NEURALE DE TRANSPLANTATION, ET FEUILLE DE RÉTINE NEURALE DE TRANSPLANTATION
专利权人:
RIKEN;SUMITOMO CHEMICAL COMPANY, LIMITED;国立研究開発法人理化学研究所;SUMITOMO DAINIPPON PHARMA CO., LTD.;住友化学株式会社;大日本住友製薬株式会社
发明人:
KUWAHARA Atsushi,桑原篤,WATARI Kenji,渡健治,MATSUSHITA Keizo,松下恵三,YAMASAKI Suguru,山▲崎▼ 優,MANDAI Michiko,万代道子,TAKAHASHI Masayo,高橋政代
申请号:
JPJP2020/011254
公开号:
WO2020/184720A1
申请日:
2020.03.13
申请国别(地区):
JP
年份:
2020
代理人:
摘要:
This method for evaluating the quality of a transplant neural retina includes: extracting, as a quality evaluation sample, a portion of or all of a cell aggregate that contains a neural retina having an epithelial structure derived from pluripotent stem cells; detecting, in the quality evaluation sample, the expressions of genes related to neural-retina-based cells and genes related to non-neural-retina-based cells; and, in the case in which the expressions of the genes related to the neural-retina-based cells are recognized and the expressions of the genes related to the non-neural-retina-based genes are not recognized, determining that it is possible to use, as the transplant neural retina, (1) a neural retina (transplant neural retina) in a cell aggregate that is the same as the cell aggregate that contains the quality evaluation sample as a portion thereof, (2) a neural retina (transplant neural retina) in a cell aggregate of the same lot as the cell aggregate that contains the quality evaluation sample as a portion thereof, or (3) a neural retina (transplant neural retina) in a cell aggregate of the same lot as the cell aggregate the entirety of which is the quality evaluation sample. The genes related to non-neural-retina-based cells are one or more types of genes selected from the group consisting of cerebrospinal tissue marker genes and eye-related tissue marker genes.L'invention porte sur un procédé d'évaluation de la qualité d'une rétine neurale de transplantation comprenant les étapes suivantes consistant à : extraire, en tant qu'échantillon d'évaluation de la qualité, une partie ou la totalité d'un agrégat cellulaire qui contient une rétine neurale possédant une structure épithéliale dérivée de cellules souches pluripotentes ; détecter, dans l'échantillon d'évaluation de la qualité, les expressions de gènes associés à des cellules à base de rétine neurale et de gènes associés à des cellules à base de rétine non neurale ; et, dans le cas où les expression
来源网站:
中国工程科技知识中心
来源网址:
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