The TT1 gene, the recombinant expression vector containing the gene, the polypeptide encoded by the gene and the use thereof are provided. The TT1 gene can effectively increase tolerance of plants and microbes to abiotic stresses, such as drought, acid-alkaline, saline-alkaline and heat. The methods for producing transgenic plants and microbes are also provided. The methods are simple and effective. The transgenic plants and microbes exhibit enhanced tolerance to environmental stresses.