The invention relates to a preparation method of recombinant human granulocyte colony stimulating factor. Specifically, the cDNA sequence with the method of the invention is formed by the human sequence mutation, the method provided by the invention can make the expression of protein (recombinant human granulocyte colony stimulating factor) increased significantly.本發明涉及一種重組人粒細胞集落刺激因子的製備方法。具體而言,本發明所述方法採用的cDNA序列是由人源序列突變而成,採用本發明提供的方法,可以使得目的蛋白即重組人粒細胞刺激因子的表達量顯著提高。
