The invention relates to novel TNF family ligand trimer-containing antigen binding molecules comprising (a) at least one antigen binding moiety capable of specific binding to Tenascin-C (TnC) and (b) a first and a second polypeptide that are linked to each other by a disulfide bond, wherein the antigen binding molecules are characterized in that the first polypeptide comprises two ectodomains of a TNF ligand family member or two fragments thereof that are connected to each other by a peptide linker and in that the second polypeptide comprises only one ectodomain of said TNF ligand family member or a fragment thereof. The invention further relates to methods of producing these molecules and to methods of using the same.本發明係關於新穎的含有TNF家族配位三聚體之抗原結合分子，其包含(a)至少一個能夠特異性結合於肌腱蛋白-C (TnC)之抗原結合部分及(b)第一及第二多肽，其藉由雙硫鍵彼此連接，其中該抗原結合分子之特徵在於第一多肽包含TNF配位體家族成員之兩個胞外域或其兩個片段，該兩個胞外域或其兩個片段藉由肽連接子彼此連接，且第二多肽包含該TNF配位體家族成員之僅一個胞外域或其片段。本發明亦係關於此等分子之製備方法及其使用方法。
