The present invention relates to a floss holder which allows floss to be conveniently and precisely operated while one side of the floss is held by hands of a user, and to be hygienically used and effectively replaced and installed. The floss holder comprises: a floss case (100); a bobbin (200) for winding the floss in the floss case (100); a rotation sawtooth (300) provided on the side of the bobbin (200) for controlling the rotation of the bobbin (200); and a control unit (400) for controlling the rotation of the bobbin (200) and the rotation sawtooth (300).본 명세서는 치실 일측만 사용자의 손으로 파지하여 편리하고 정확하게 치실을 조작할 수 있으면서 동시에, 위생적인 사용과 효과적인 치실 교체 장착이 가능한 치실 홀더에 관한 것이다.