PURPOSE: A tube type cell culture scaffold and a producing apparatus of the same are provided to be able to produce a scaffold in more various types, and simply implement a structure of a collecting unit and a fixed axis so as to facilitate processing of the collecting unit, considering the characteristics of a scaffold for cell culture that the diameter is small. CONSTITUTION: A tube type cell culture scaffold is produced in a tube type, with two or more overlapping layers in a radial direction, of which each layer has a different form from each other. The tube type cell culture scaffold includes a first type ejected material that is formed by a biological adaptation material forming a scaffold is ejected in a radius direction continuously or discontinuously; a second type ejected material that is formed into a straight line form in a longitudinal direction, while being repetitively formed in the radius direction being space apart from each other; and a third type ejected material that is formed into a film form to culture cells not mixing endothelial cells and perithelial cells. The tube type cell culture scaffold is characterized that a mesh type ejected material that is formed by contacts and overlapping of the first type ejected material and the second type ejected material while contacting and overlapping to the inner sides or outer sides of the third type ejected material in the radius direction.본 발명은 세포배양지지체 제조 장치 및 이를 이용하여 제조된 세포배양지지체에 관한 것으로서, 더욱 상세하게 세포배양지지체를 생성하기 위한 용액이 분사되는 분사수단 하부에 구비되는 수집부가 원통형으로 형성되되, 상기 수집부와 상기 수집부를 고정하는 고정축에 있어서, 상기 고정축의 단부가 원뿔 형태로 형성되고, 상기 수집부의 양측 단부가 상기 고정축에 대응되는 형태로 내측으로 파여져 형성됨으로써, 상기 수집부 및 고정축의 구조가 간단하여 가공 및 결합과정이 간편한 세포배양지지체 제조 장치 및 이를 이용하여 다양한 형태로 제조된 세포배양지지체에 관한 것이다.