METHODS FOR PREPARATION OF PURIFIED CARDIOMYOCYTE CELL MASSES DERIVED FROM MULTIPOTENT STEM CELLS AND A FILM OF CARDIOMYOCYTE CELL MASSES AS WELL AS MEDICAL DEVICE AND USE OF CELLULAR MASSES
"Methods of preparing purified cardiomyocyte cell masses derived from pluripotent stem cells and a cardiomyocyte cell mass film, as well as medical device and use of said cell masses". The present invention relates to improving post-transplantation graft rate of cardiomyocytes that have been purified to such an extent that they are free of non-cardiomyocytes and any components derived from other species. To solve the problem, the present inventors studied the possibility of building purified cardiomyocyte cell masses. As a result, they showed that the stated problem could be solved by providing a method of preparing pluripotent stem cell-derived cardiomyocyte cell masses, characterized in that the cell masses of aggregated cells containing the cardiomyocytes that were differentiated and induced by from pluripotent stem cells, which were dispersed as isolated cells to thereby obtain purified cardiomyocytes, which were then cultured in a culture medium under serum-free conditions, could be reassembled.patente de invenção: "métodos de preparação de massas celulares de cardiomiócitos purificados derivados de células-tronco pluripotentes e de uma película de massas celulares de cardiomiócitos, bem como dispositivo médico e uso das referidas massas celulares". a presente invenção refere-se a melhorara da taxa de enxertia pós-transplante de cardiomiócitos que foram purificados em tal proporção que eles estão livres de não-cardiomiócitos e quaisquer componentes derivados de outras espécies. para solucionar o problema, os presentes inventores estudaram a possibilidade de construir massas celulares de cardiomiócitos purificados. como resultado, eles mostraram que o problema declarado poderia ser solucionado pelo fornecimento de um método de preparação de massas celulares de cardiomiócitos derivados de célulastronco pluripotentes, caracterizado pelo fato de que as massas celulares de células agregadas que contêm os cardiomiócitos que foram diferenciados e induzidos a partir de
