In the present invention, a tissue culturing method is disclosed and which comprises following steps: providing a chopped gametophyte, generating calluses by culturing the chopped gametophyte, and apogamic regenerating sporophytes, by culturing the calluses in a liquid medium to develop the sporophytes from the calluses. Furthermore, the present invention also provides a tissue culturing method of ferns and an explant.本發明係關於一種組織培養方法,包含:提供一碎裂的配子體;增生一癒傷組織,係培養該碎裂的配子體,使該碎裂的配子體增生該癒傷組織;經無配生殖再生一孢子體,係培養該癒傷組織於一培養液中,使該癒傷組織再生該孢子體。此外,本發明另提供一蕨類培養方法及一植物培植體。S1-S5‧‧‧步驟
