This invention provides a blood irradiation apparatus with a single X-ray source and an irradiation method thereof, which are applied to the technical field of blood irradiators. The blood irradiation apparatus includes a framework and an X-ray tube disposed on the framework. A blood bag receiver is arranged below the X-ray tube and is composed of a bottom plate and an outer wall and an inner wall that are arranged on the bottom plate. An annular blood bag accommodating cavity is enclosed between the outer and inner walls and is used for placing blood or blood products to be irradiated. The bottom plate of the blood bag receiver is movably disposed on the framework via a rotating shaft, and the rotating shaft is connected to an output shaft of a rotating motor via a transmission. According to this invention, the X-rays generated by the X-ray tube are made full use of, so that the utilization rate is improved. In addition, a rotating motor is used to rotate the blood bag receiver back and forth to force the blood or blood product in each blood bag to flow about, so that the blood or blood product in various local regions can exchange their positions with one another, thereby improving the uniformity of radiation of the blood or blood product.
