LEE, KUNG TA,李昆达,李昆達,HSIEH, SHAN CHI,谢善棋,謝善棋,SU, CHING YEUH,苏敬岳,蘇敬岳,LAI, YU CHEN,来宥丞,來宥丞
申请号：
TW107109303
公开号：
TW201938185A
申请日:
2018.03.19
申请国别(地区):
TW
年份:
2019
代理人:
摘要：
Provided is use of a protein encoded by a nucleotide sequence with 90% or higher sequence identity to SEQ ID NO:1 for regulation of gene expression. The protein activates expression of a gene under induction of a medium or long chain alkane or a medium or long chain fatty acid methyl ester. Also provided is a whole cell catalytic system under regulation of a medium or long chain alkane or a medium or long chain fatty acid methyl ester. The system includes a recombinant microorganism cell expressing the protein and an alkane monooxygenase. Also provided is a method of preparing a terminal oxidation product of a medium or long chain alkane using the whole cell catalytic system.本發明提供一種與SEQ ID NO：1有90%以上序列同一性之核苷酸序列所編碼的蛋白質用於調控基因表現的用途，該蛋白質在一中鏈或長鏈烷類或一中鏈或長鏈脂肪酸甲酯的誘導下活化一基因之表現。本發明亦提供一種受中鏈或長鏈烷類或中鏈或長鏈脂肪酸甲酯調控之全細胞催化系統，該系統包含表現該蛋白質及一烷類單加氧酶的一重組微生物細胞。本發明亦提供利用該全細胞催化系統的一種中鏈或長鏈烷類末端氧化產物的製備方法。
