INTÉGRATION SPÉCIFIQUE À UN SITE D'UN TRANSGÈNE AU MOYEN D'UNE RECOMBINAISON INTRAGÉNOMIQUE PAR L'INTERMÉDIAIRE D'UNE VOIE DE RÉPARATION PAR JONCTION D'EXTRÉMITÉ NON HOMOLOGUE
KUMAR, Sandeep,PETOLINO, Joseph F.,WORDEN, Andrew F.,BARONE, Pierluigi,SIMPSON, Matthew A.,STRANGE, Tonya L.
申请号：
EP17871193
公开号：
EP3541168A4
申请日:
2017.10.30
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
Compositions and methods to modify at least one target locus in a plant cell are provided, which comprises providing a plant cell, a plant, or a plant part with one or more target loci and one or more donor loci, providing at least one cleaving site specific nuclease to produce a double strand break within the target loci, followed by non-homologous end joining of at least one donor locus within at least one target locus. Target loci, donor loci and nuclease loci used in these methods, and plant cells, plants and plant parts comprising these target loci, donor loci, nuclease loci and/or the recombined loci are also provided.
