The invention provides a method of increasing blood flow or perfusion in an ischemic tissue； inducing angiogenesis, neovascularization or revascularization； increasing skeletal muscle viability； promoting ischemic skin wound healing； treating or preventing gangrene； and/or treating CLI. In various aspects, the method comprises administering to a subject a hybrid adenoassociated virus (AAV) comprising a nucleotide sequence encoding an E-selectin, AAV serotype 2 (AAV2) inverted terminal repeats (ITRs), and a capsid from an AAV other than serotype 2. In various aspects, the method comprises administering to the subject a cell comprising an AAV comprising a nucleotide sequence encoding an E-selectin, AAV2 ITRs, and a AAV2 capsid.
